Confocal microscopy is a powerful technique for acquiring three-dimensional images of biological samples. The video describes the basic principles of confocal microscopy, with specific discussions of the operation of laser scanning and spinning disk confocal microscopes and of their application to biology. The examples are not neural, but the basic principles of the technique are explained clearly. [Description modified from the iBioSeminars website: http://www.ibioseminars.org/.]