This purkinje neuron from a mouse cerebellum was imaged with the fluorescent dye Alexa 594, injected into the cell with a microelectrode. The high resolution image was captured with a two photon laser scanning microscope. The microscope took two dimensional images of the neuron, and then advanced deeper into the tissue, one micron at a time, taking many more scans. The computer took the stack of images and reconstructed the 3D video you see here.